CASP9 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CASP9.
Immunogen
A synthetic peptide corresponding to amino acids 280-320 of human CASP9.
Host
Rabbit
Theoretical MW (kDa)
~35, 48
Reactivity
Human
Specificity
CASP9 polyclonal antibody detects endogenous levels of CASP9 protein.
Form
Liquid
Purification
Antigen affinity purification
Purity
> 95% by SDS-PAGE
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In 1x PBS, pH 7.2 (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with CASP9 polyclonal antibody (Cat # PAB24855).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using CASP9 polyclonal antibody (Cat # PAB24855).Immunofluorescence
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Gene Info — CASP9
Entrez GeneID
842Protein Accession#
P55211Gene Name
CASP9
Gene Alias
APAF-3, APAF3, CASPASE-9c, ICE-LAP6, MCH6
Gene Description
caspase 9, apoptosis-related cysteine peptidase
Omim ID
602234Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq
Other Designations
ICE-like apoptotic protease 6|OTTHUMP00000002322|OTTHUMP00000002323|OTTHUMP00000044594|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9|caspase 9, apoptosis-related cysteine protease
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Interactomes
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Pathways
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Diseases
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