SPP1 polyclonal antibody
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More Files
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Western Blot (Recombinant protein)
Immunoblotting of SPP1 polyclonal antibody (Cat # PAB10297) was used at a 1:1,000 dilution to detect SPP1 by Western blot.
In lane 1, reactivity is shown against 250 ng of human SPP1.
Lane 2 shows reactivity of MMP-cleaved SPP1.
Use a 1 : 10,000 dilution of HRP conjugated Gt-a-Rabbit IgG for detection.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of SPP1 polyclonal antibody (Cat # PAB10297) was used at a 1 : 100-1 : 300 dilution to detect SPP1 by immunohistochemistry.
SPP1 is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells).
There is also weak staining of the extracellular matrix.
SPP1 is not expressed in breast tumor cells, and there is no staining of the breast cells in this section.
No antigen retrieval is required.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SPP1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human SPP1.
Host
Rabbit
Theoretical MW (kDa)
66, 32
Reactivity
Dog, Human, Pig
Specificity
This antibody recognizes the full-length osteopontin protein (which runs at 66 KDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 KDa MMP-cleaved C-fragment is recognized, but not the 40 KDa N-terminal fragment.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:20000-1:100000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:100-1:300)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Immunoblotting of SPP1 polyclonal antibody (Cat # PAB10297) was used at a 1:1,000 dilution to detect SPP1 by Western blot.
In lane 1, reactivity is shown against 250 ng of human SPP1.
Lane 2 shows reactivity of MMP-cleaved SPP1.
Use a 1 : 10,000 dilution of HRP conjugated Gt-a-Rabbit IgG for detection.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of SPP1 polyclonal antibody (Cat # PAB10297) was used at a 1 : 100-1 : 300 dilution to detect SPP1 by immunohistochemistry.
SPP1 is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells).
There is also weak staining of the extracellular matrix.
SPP1 is not expressed in breast tumor cells, and there is no staining of the breast cells in this section.
No antigen retrieval is required.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SPP1
Entrez GeneID
6696Gene Name
SPP1
Gene Alias
BNSP, BSPI, ETA-1, MGC110940, OPN
Gene Description
secreted phosphoprotein 1
Omim ID
166490Gene Ontology
HyperlinkGene Summary
bone sialoprotein I
Other Designations
SPP1/CALPHA1 fusion|bone sialoprotein I|early T-lymphocyte activation 1|osteopontin|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein|secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
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Interactome
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Pathway
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Disease
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Publication Reference
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The immunology of Eta-1/osteopontin.
Weber GF, Cantor H.
Cytokine & Growth Factor Reviews 1996 Oct; 7(3):241.
Application：ELISA, IHC, WB-Ce, WB-Ti, Human, Rat, B lymphocytes, Hearts, Lungs, Macrophages, Monocytes, Natural killer cells, Skeletal muscles, Skin, T lymphocytes.
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Osteopontin-induced modifications of cellular functions.
Denhardt DT, Lopez CA, Rollo EE, Hwang SM, An XR, Walther SE.
Annals of the New York Academy of Sciences 1995 Apr; 56(6):1367.
Application：WB-Re, N/A, Recombinant protein.
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Overcoming obstacles to metastasis--defenses against host defenses: osteopontin (OPN) as a shield against attack by cytotoxic host cells.
Denhardt DT, Chambers AF.
Journal of Cellular Biochemistry 1994 Sep; 56(1):48.
Application：ELISA, Human, Mouse, Cancer cells, Human plasma, HUVECs, Macrophages, NIH/3T3 cells.
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The immunology of Eta-1/osteopontin.
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