SPP1 recombinant monoclonal antibody, clone 10A1
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SPP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human SPP1.
Theoretical MW (kDa)
Calculated MW: 36, 3
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 293T whole cell lysate, U-251 whole cell lysate, U-87 whole cell lysate with SPP1 recombinant monoclonal antibody, clone 10A1 (Cat # RAB07486).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using SPP1 recombinant monoclonal antibody, clone 10A1 (Cat # RAB07486) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SPP1
Entrez GeneID
6696Protein Accession#
P10451Gene Name
SPP1
Gene Alias
BNSP, BSPI, ETA-1, MGC110940, OPN
Gene Description
secreted phosphoprotein 1
Omim ID
166490Gene Ontology
HyperlinkGene Summary
bone sialoprotein I
Other Designations
SPP1/CALPHA1 fusion|bone sialoprotein I|early T-lymphocyte activation 1|osteopontin|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein|secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
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Interactome
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Pathway
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Disease
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