SPP1 recombinant monoclonal antibody, clone R08-1C5
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SPP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human Osteopontin.
Theoretical MW (kDa)
Calculated MW: 35 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Osteopontin in Hela, CHO-K1, C6, Jurkat lysates using Osteopontin antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Osteopontin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Gene Info — SPP1
Entrez GeneID
6696Protein Accession#
P10451Gene Name
SPP1
Gene Alias
BNSP, BSPI, ETA-1, MGC110940, OPN
Gene Description
secreted phosphoprotein 1
Omim ID
166490Gene Ontology
HyperlinkGene Summary
bone sialoprotein I
Other Designations
SPP1/CALPHA1 fusion|bone sialoprotein I|early T-lymphocyte activation 1|osteopontin|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein|secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
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Interactome
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Pathway
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Disease
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