MAP3K7 monoclonal antibody (M02), clone 2E10
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Specifications
Product Description
Mouse monoclonal antibody raised against a full length recombinant MAP3K7.
Immunogen
MAP3K7 (AAH17715, 1 a.a. ~ 579 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSTASAASSSSSSSAGEMIEAPSQVLNFEEIDYKEIEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESESERKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVMEYAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQGVAYLHSMQPKALIHRDLKPPNLLLVAGGTVLKICDFGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKCDVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHNGTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEIVKIMTHLMRYFPGADEPLQYPCQYSDEGQSNSATSTGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLESKLLKNQAKQQSESGRLSLGASRGSSVESLPPTSEGKRMSADMSEIEARIAATTGNGQPRRRSIQDLTVTGTEPGQVSSRSSSPSVRMITTSGPTSEKPTRSHPWTPDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSKESMAVFEQHCKMAQEYMKVQTEIALLLQRKQELVAELDQDEKDQQNTSRLVQEHKKLLDENKSLSTYYQQCKKQLEVIRSQQQKRQGTS
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MAP3K7 expression in transfected 293T cell line by MAP3K7 monoclonal antibody (M02), clone 2E10.
Lane 1: MAP3K7 transfected lysate(63.8 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAP3K7 is approximately 1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of MAP3K7 over-expressed 293 cell line, cotransfected with MAP3K7 Validated Chimera RNAi ( Cat # H00006885-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAP3K7 monoclonal antibody (M02) clone 2E10 (Cat # H00006885-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.RNAi Knockdown (Antibody validated)
Western blot analysis of MAP3K7 over-expressed 293 cell line, cotransfected with MAP3K7 Validated Chimera RNAi ( Cat # H00006885-R02V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAP3K7 monoclonal antibody (M02) clone 2E10 (Cat # H00006885-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — MAP3K7
Entrez GeneID
6885GeneBank Accession#
BC017715Protein Accession#
AAH17715Gene Name
MAP3K7
Gene Alias
TAK1, TGF1a
Gene Description
mitogen-activated protein kinase kinase kinase 7
Omim ID
602614Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
OTTHUMP00000016870|OTTHUMP00000016871|OTTHUMP00000016872|OTTHUMP00000016873|TGF-beta activated kinase 1|transforming growth factor-beta-activated kinase 1
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Interactomes
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