SPP1 monoclonal antibody (M11), clone 2D12
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Specifications
Product Description
Mouse monoclonal antibody raised against a full length recombinant SPP1.
Immunogen
SPP1 (AAH07016.1, 1 a.a. ~ 300 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MRIAVICFCLLGITCAIPVKQADSGSSEEKQLYNKYPDAVATWLNPDPSQKQNLLAPQTLPSKSNESHDHMDDMDDEDDDDHVDSQDSIDSNDSDDVDDTDDSHQSDESHHSDESDELVTDFPTDLPATEVFTPVVPTVDTYDGRGDSVVYGLRSKSKKFRRPDIQYPDATDEDITSHMESEELNGAYKAIPVAQDLNAPSDWDSRGKDSYETSQLDDQSAETHSHKQSRLYKRKANDESNEHSDVIDSQELSKVSREFHSHEFHSHEDMLVVDPKSKEEDKHLKFRISHELESASSEVN
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (58.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
SPP1 monoclonal antibody (M11), clone 2D12. Western Blot analysis of SPP1 expression in human stomach.Western Blot (Cell lysate)
SPP1 monoclonal antibody (M11), clone 2D12 Western Blot analysis of SPP1 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Cell lysate)
SPP1 monoclonal antibody (M11), clone 2D12. Western Blot analysis of SPP1 expression in COLO 320 HSR ( Cat # L020V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SPP1 is approximately 3ng/ml as a capture antibody.ELISA
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Gene Info — SPP1
Entrez GeneID
6696GeneBank Accession#
BC007016Protein Accession#
AAH07016.1Gene Name
SPP1
Gene Alias
BNSP, BSPI, ETA-1, MGC110940, OPN
Gene Description
secreted phosphoprotein 1
Omim ID
166490Gene Ontology
HyperlinkGene Summary
bone sialoprotein I
Other Designations
SPP1/CALPHA1 fusion|bone sialoprotein I|early T-lymphocyte activation 1|osteopontin|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein|secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Polydatin, Natural Precursor of Resveratrol, Promotes Osteogenic Differentiation of Mesenchymal Stem Cells.
Di Benedetto A, Posa F, De Maria S, Ravagnan G, Ballini A, Porro C, Trotta T, Grano M, Muzio LL, Mori G.
International Journal of Medical Sciences 2018 Jun; 15(9):944.
Application:WB, Human, Human dental bud stem cells.
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Polydatin, Natural Precursor of Resveratrol, Promotes Osteogenic Differentiation of Mesenchymal Stem Cells.
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