CFL1 recombinant monoclonal antibody, clone R06-2C0
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Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against human CFL1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human CFL1.
Theoretical MW (kDa)
Calculated MW: 19 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of A549, MCF-7, 293T lysates with CFL1 recombinant monoclonal antibody, clone R06-2C0 (Cat # RAB01467).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cofilin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunofluorescence
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Gene Info — CFL1
Entrez GeneID
1072Protein Accession#
P23528Gene Name
CFL1
Gene Alias
CFL
Gene Description
cofilin 1 (non-muscle)
Omim ID
601442Gene Ontology
HyperlinkGene Summary
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM
Other Designations
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