IGF1 (Human) Recombinant Protein
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Specifications
Product Description
Human IGF1 (NP_000609, 49 a.a. - 118 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
MGPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA
Host
Escherichia coli
Theoretical MW (kDa)
7.7
Form
Liquid
Preparation Method
Escherichia coli expression system
Concentration
1 mg/mL
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1.0 EU per 1 ug of protein (determined by LAL method).
Activity
The ED50 for this effect is < 5 ng/mL. Measured in a cell proliferation assay using MCF7 cell.
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In PBS, pH 7.4.
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — IGF1
Entrez GeneID
3479Protein Accession#
NP_000609; P05019Gene Name
IGF1
Gene Alias
IGFI
Gene Description
insulin-like growth factor 1 (somatomedin C)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene
Other Designations
insulin-like growth factor 1|somatomedin C
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