EGF (Human) Recombinant Protein
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Specifications
Product Description
Human EGF (NP_001954, 971 a.a. - 1023 a.a.) partial recombinant protein expressed in Escherichia coli.
Sequence
MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR
Host
Escherichia coli
Theoretical MW (kDa)
6.3
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Conventional Chromatography
Concentration
1 mg/mL
Purity
> 95% by SDS-PAGE
Activity
The ED50 for this effect is < 0.89 ng/mL . Measured in a cell proliferation assay using mouse Balb3T3 cell.
Quality Control Testing
Loading 3 ug protein in 15% SDS-PAGE
Storage Buffer
In PBS, pH 7.4.
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — EGF
Entrez GeneID
1950Protein Accession#
NP_001954Gene Name
EGF
Gene Alias
HOMG4, URG
Gene Description
epidermal growth factor (beta-urogastrone)
Omim ID
131530Gene Ontology
HyperlinkGene Summary
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq
Other Designations
urogastrone
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Interactomes
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Pathways
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Diseases
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