Anti-Trastuzumab monoclonal antibody, clone 5A4-11
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Specifications
Product Description
Mouse monoclonal antibody raised against F(ab)2 fragment of Trastuzumab.
Target gene is HER2/neu.Immunogen
F(ab)2 fragment of Trastuzumab.
Host
Mouse
Specificity
This antibody is able to interfere the binding between Trastuzumab and HER2/neu-expressing BT-474 cells.
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA (2 ug/mL for coating)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 8.0 (0.09% sodium azide).
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ERBB2
Entrez GeneID
2064Gene Name
ERBB2
Gene Alias
CD340, HER-2, HER-2/neu, HER2, NEU, NGL, TKR1
Gene Description
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq
Other Designations
c-erb B2/neu protein|erbB-2|herstatin|neuroblastoma/glioblastoma derived oncogene homolog|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Bioluminescent Bridging Immunoassay for Anti-Drug Antibody (ADA) Detection.
Nidhi Nath, Becky Godat, Rod Flemming, Marjeta Urh.
Methods in Molecular Biology (Clifton, N.J.) 2022 Jan; 2313:313.
Application:EIA, Human, Human serum, Recombinant proteins.
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Antibody drug quantitation in coexistence with anti-drug antibodies on nSMOL bioanalysis.
Iwamoto N, Hamada A, Shimada T.
Analytical Biochemistry 2017 Nov; 540-541:30.
Application:Func, ELISA (Trastuzumab ).
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Development of NanoLuc bridging immunoassay for detection of anti-drug antibodies.
Nath N, Flemming R, Godat B, Urh M.
Journal of Immunological Methods 2017 Jul; 450:17.
Application:Func, Human, Human serum.
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Bioluminescent Bridging Immunoassay for Anti-Drug Antibody (ADA) Detection.
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