SQSTM1 monoclonal antibody (M03), clone 1C9
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More Files
- More Functions
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SQSTM1 is approximately 3ng/ml as a capture antibody.
Western Blot detection against Immunogen (74.14 KDa) .
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Specifications
Product Description
Mouse monoclonal antibody raised against a full-length recombinant SQSTM1.
Immunogen
SQSTM1 (AAH03139.1, 1 a.a. ~ 440 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (90); Rat (90)
Isotype
IgG3 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (74.14 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SQSTM1 is approximately 3ng/ml as a capture antibody.ELISA
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Gene Info — SQSTM1
Entrez GeneID
8878GeneBank Accession#
BC003139Protein Accession#
AAH03139.1Gene Name
SQSTM1
Gene Alias
A170, OSIL, PDB3, ZIP3, p60, p62, p62B
Gene Description
sequestosome 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq
Other Designations
EBI3-associated protein p60|Paget disease of bone 3|oxidative stress induced like|phosphotyrosine independent ligand for the Lck SH2 domain p62|ubiquitin-binding protein p62
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Interactomes
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Diseases
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Publication Reference
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ADH5-mediated NO bioactivity maintains metabolic homeostasis in brown adipose tissue.
Sara C Sebag, Zeyuan Zhang, Qingwen Qian, Mark Li, Zhiyong Zhu, Mikako Harata, Wenxian Li, Leonid V Zingman, Limin Liu, Vitor A Lira, Matthew J Potthoff, Alexander Bartelt, Ling Yang.
Cell Reports 2021 Nov; 37(7):110003.
Application：WB-Ce, Mouse, Mouse brown adipose tissue.
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The Unfolded Protein Response Regulates Hepatic Autophagy by sXBP1-mediated Activation of TFEB.
Zeyuan Zhang, Qingwen Qian, Mark Li, Fan Shao, Wen-Xing Ding, Vitor A Lira, Sophia X Chen, Sara C Sebag, Gökhan S Hotamisligil, Huojun Cao, Ling Yang.
Autophagy 2020 Jul; 1.
Application：WB-Ti, WB-Tr, Mouse, Mouse hepatocytes, Mouse liver.
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Hepatic Lysosomal iNOS Activity Impairs Autophagy in Obesity.
Qian Q, Zhang Z, Li M, Savage K, Cheng D, Rauckhorst AJ, Ankrum JA, Taylor EB, Ding WX, Xiao Y, Cao HJ, Yang L.
Cellular and Molecular Gastroenterology and Hepatology 2019 Mar; 8(1):95.
Application：WB-Ce, Mouse, Mouse hepatocytes.
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The flavonoid TL-2-8 induces cell death and immature mitophagy in breast cancer cells via abrogating the function of the AHA1/Hsp90 complex.
Liu HJ, Jiang XX, Guo YZ, Sun FH, Kou XH, Bao Y, Zhang ZQ, Lin ZH, Ding TB, Jiang L, Lei XS, Yang YH.
Acta Pharmacologica Sinica 2017 May; [Epub].
Application：WB-Ce, Human, cell lysate of MDA-MB-231, MDA-MB-468, MCF-7 cells.
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IKKα controls ATG16L1 degradation to prevent ER stress during inflammation.
Diamanti MA, Gupta J, Bennecke M, De Oliveira T, Ramakrishnan M, Braczynski AK, Richter B, Beli P, Hu Y, Saleh M, Mittelbronn M, Dikic I, Greten FR.
The Journal of Experimental Medicine 2017 Feb; 214(2):423.
Application：WB, Mouse, Mouse intestinal epithelial cells (IECs).
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Tau-driven 26S proteasome impairment and cognitive dysfunction can be prevented early in disease by activating cAMP-PKA signaling.
Myeku N, Clelland CL, Emrani S, Kukushkin NV, Yu WH, Goldberg AL, Duff KE.
Nature Medicine 2016 Jan; 22(1):46.
Application：IF, WB-Ti, Mouse, Cortical.
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Hypoxia counteracts taxol-induced apoptosis in MDA-MB-231 breast cancer cells: role of autophagy and JNK activation.
Notte A, Ninane N, Arnould T, Michiels C.
Cell Death & Disease 2013 May; 4:e638.
Application：WB-Ce, WB-Tr, Human, MDA-MB-231 cells.
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BNIP3 protects HepG2 cells against etoposide-induced cell death under hypoxia by an autophagy-independent pathway.
Cosse JP, Rommelaere G, Ninane N, Arnould T, Michiels C.
Biochemical Pharmacology 2010 Oct; 80(8):1160.
Application：WB, Human, HepG2 cells.
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A Role for NBR1 in Autophagosomal Degradation of Ubiquitinated Substrates.
Kirkin V, Lamark T, Sou YS, Bjorkoy G, Nunn JL, Bruun JA, Shvets E, McEwan DG, Clausen TH, Wild P, Bilusic I, Theurillat JP, Overvatn A, Ishii T, Elazar Z, Komatsu M, Dikic I, Johansen T.
Molecular Cell 2009 Feb; 33(4):505.
Application：WB, IF, IHC, Human, Mouse, HeLa cells, liver.
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ADH5-mediated NO bioactivity maintains metabolic homeostasis in brown adipose tissue.
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