KRT8 monoclonal antibody (M02), clone 3E3

Catalog # H00003856-M02

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Size:100 ug
Price: USD $ 335.00
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  • +1-909-264-1399
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    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Transfected lysate)
Application

Western Blot (Transfected lysate)

Western Blot analysis of KRT8 expression in transfected 293T cell line by KRT8 monoclonal antibody (M02), clone 3E3.

Lane 1: KRT8 transfected lysate(53.7 KDa).
Lane 2: Non-transfected lysate.

Sandwich ELISA (Recombinant protein)
Application

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged KRT8 is approximately 1ng/ml as a capture antibody.

Immunofluorescence
Application

Immunofluorescence

Immunofluorescence of monoclonal antibody to KRT8 on HeLa cell . [antibody concentration 10 ug/ml]

QC Test

Western Blot detection against Immunogen (37.29 KDa) .

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against a partial recombinant KRT8.

    Immunogen

    KRT8 (NP_002264, 91 a.a. ~ 195 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

    Sequence

    EKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVL

    Host

    Mouse

    Reactivity

    Human

    Isotype

    IgG2b Kappa

    Quality Control Testing

    Antibody Reactive Against Recombinant Protein.

    Western Blot detection against Immunogen (37.29 KDa) .

    Storage Buffer

    In 1x PBS, pH 7.4

    Storage Instruction

    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot (Transfected lysate)

    Western Blot analysis of KRT8 expression in transfected 293T cell line by KRT8 monoclonal antibody (M02), clone 3E3.

    Lane 1: KRT8 transfected lysate(53.7 KDa).
    Lane 2: Non-transfected lysate.

    Western Blot (Recombinant protein)

    Sandwich ELISA (Recombinant protein)

    Detection limit for recombinant GST tagged KRT8 is approximately 1ng/ml as a capture antibody.

    ELISA

    Immunofluorescence

    Immunofluorescence of monoclonal antibody to KRT8 on HeLa cell . [antibody concentration 10 ug/ml]
  • Gene Info — KRT8

    Entrez GeneID

    3856

    GeneBank Accession#

    NM_002273

    Protein Accession#

    NP_002264

    Gene Name

    KRT8

    Gene Alias

    CARD2, CK8, CYK8, K2C8, K8, KO

    Gene Description

    keratin 8

    Omim ID

    148060 215600

    Gene Ontology

    Hyperlink

    Gene Summary

    This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq

    Other Designations

    cytokeratin 8|keratin, type II cytoskeletal 8

  • Interactomes
  • Diseases
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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