Abnova provides a total solution for ELISA kit service encompassing antibody production, antibody pairing, sandwich ELISA optimization, ELISA kit manufacturing, documentation, and certifications. Abnova can produce the antibodies for the customer; the customer can also provide their own generated antibodies. Polyclonal, monoclonal, and alternate species antibodies are useful for antibody pair development. Customers may request detecting antibody conjugation or use pre-conjugated secondary antibody. Sandwich ELISA optimization including antibody pair, blocker, diluent, and stability selection and their concentrations have a significant impact on the final ELISA kit sensitivity and specificity. Factors to consider for ELISA kit manufacturing include capture antibody scaled-up and ELISA plate coating, detecting antibody scaled-up versus “ready-to-use” format, and overall kit stability and storage conditions.

Flowchart

 
 

Antibody Production

  • Abnova’s Antibodies
  • Customer’s Antibodies
 
 

Antibody Pairing

  • Capture vs. Detecting Antibodies
  • Polyclonal vs. Monoclonal Antibodies
  • Different Species Antibodies
  • Detecting Antibody Conjugation (Biotin, HRP)
  • Secondary Antibodies (HRP)
 
 

Sandwich ELISA Optimization

  • Capture and Detecting Antibody Concentration
  • Blocker Selection and Concentration
  • Diluent Selection and Concentration
  • Stabilizer Selection and Concentration
  • Sensitivity and Specificity
 
 

ELISA Kit Manufacturing

  • Capture Antibody Scaled-up and ELISA Plate Coating
  • Detecting Antibody Scaled Up
  • Detecting Antibody “Ready-to-Use”
  • ELISA Kit Stability and Storage
 
 

Documentations and Certifications

  • ISO13485
  • GMP
  • CE-IVD
  • US EUA and IVD
 
 

Example

  • Abnova COVID-19 Neutralizing Antibody ELISA Kit: A rapid, simple, and sensitive method to characterize the binding affinity of the RBD-ACE2 complex in the presence of potential inhibitors

 
 

ELISA Related Services

For any inquiry, please contact : OEM@abnova.com