Siglec1 recombinant monoclonal antibody, clone 3D6.112 (recombinant version)
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More Files
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Flow Cytometry
Flow cytometric analysis of murine macrophages with Siglec1 recombinant monoclonal antibody, clone 3D6.112 (Cat # RAB03171).
Murine bone marrow-derived macrophages (BMDMs) were stained with RAB03171 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor® 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control- a CD169-positive population is apparent.
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Specification
Product Description
Rat recombinant monoclonal antibody raised against mouse Siglec1.
Antibody Species
Rat
Immunogen
Original antibody is raised against thioglycollate-elicted murine peritoneal macrophages (TPM) that had been cutured in mouse serum to induce sheep erythrocyte receptor (SER).
Reactivity
Mouse
Form
Liquid
Isotype
IgG2a kappa
Recommend Usage
Flow Cytometry
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Flow Cytometry
Flow cytometric analysis of murine macrophages with Siglec1 recombinant monoclonal antibody, clone 3D6.112 (Cat # RAB03171).
Murine bone marrow-derived macrophages (BMDMs) were stained with RAB03171 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor® 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control- a CD169-positive population is apparent. -
Gene Info — Siglec1
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