Isotype control recombinant monoclonal antibody, clone KLH-G9
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human Isotype control.
Antibody Species
Rabbit
Immunogen
Keyhole limpet hemocyanin carrier protein.
Form
Liquid
Purification
Protein A+G
Isotype
Rabbit IgG1k
Recommend Usage
ELISA
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
1X PBS, 0.02% Sodium azide, 50% Glycerol, 0.1% BSA
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Daudi cells untreated or treated with 200 ng/mL IL-4 using isotype control antibody KLH-G9 or using Phospho-Stat6 (Y641) antibody Stat6Y641-G12 both tested at 0.01 ug/mL.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or treated with imatinib (grey) or with pervanadate (orange) using isotype control antibody KLH-G9 compared to imatinib (red) or pervanadate (green) using Phospho-Shp2 (Y580) antibody Shp2Y580-4A2 both tested at 0.1 ug/mL.
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