KIF11 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of NIH3T3 (A), MEF (B), Hela (C), A549 (D), HL60 (E), U2OS (F), C6 (G) whole cell lysates with KIF11 recombinant monoclonal antibody (Cat # RAB02589).
Immunofluorescence
Immunofluorescent analysis of HeLa cells with KIF11 recombinant monoclonal antibody (Cat # RAB02589). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human KIF11.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein of human KIF11.
Theoretical MW (kDa)
120
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of EG5 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NIH3T3 (A), MEF (B), Hela (C), A549 (D), HL60 (E), U2OS (F), C6 (G) whole cell lysates with KIF11 recombinant monoclonal antibody (Cat # RAB02589).Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of HeLa cells with KIF11 recombinant monoclonal antibody (Cat # RAB02589). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. -
Gene Info — KIF11
Entrez GeneID
3832Protein Accession#
P52732Gene Name
KIF11
Gene Alias
EG5, HKSP, KNSL1, TRIP5
Gene Description
kinesin family member 11
Omim ID
148760Gene Ontology
HyperlinkGene Summary
This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq
Other Designations
OTTHUMP00000020099|kinesin-like 1|kinesin-like spindle protein|kinesin-related motor protein|thyroid receptor interacting protein 5
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Interactome
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Disease
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