PPP1CB recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PPP1CB.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human PPP1CB.
Theoretical MW (kDa)
36
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of PP1 beta protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of K562 (A), rat brain (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates with PPP1CB recombinant monoclonal antibody (Cat # RAB02532).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human colon cancer formalin fixed paraffin embedded tissue section using PPP1CB recombinant monoclonal antibody (Cat # RAB02532). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.61). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of HeLa cells with PPP1CB recombinant monoclonal antibody (Cat # RAB02532). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.Immunoprecipitation
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Gene Info — PPP1CB
Entrez GeneID
5500Protein Accession#
P62140Gene Name
PPP1CB
Gene Alias
MGC3672, PP-1B, PPP1CD
Gene Description
protein phosphatase 1, catalytic subunit, beta isoform
Omim ID
600590Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
protein phosphatase 1, catalytic subunit, beta|protein phosphatase 1, catalytic subunit, delta isoform|protein phosphatase 1-beta|protein phosphatase 1-delta|serine/threonine protein phosphatase PP1-beta catalytic subunit
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Interactome
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Disease
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