PPP1CB recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PPP1CB.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against synthetic peptide, corresponding to human PPP1CB.
Theoretical MW (kDa)
37
Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous levels of PPP1CB protein.
Form
Liquid
Purification
Affinity chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Concentration
0.3 mg/ml
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of K562, rat Brain, C6, 3T3, Hela lysates with PPP1CB recombinant monoclonal antibody (Cat # RAB02628).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded Human colon cancer using PPP1CB recombinant monoclonal antibody (Cat # RAB02628). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry
Immunocytochemistry
Immunocytochemical staining of human HeLa cells using PPP1CB recombinant monoclonal antibody (Cat # RAB02628)(green). The nuclear counter stain is DAPI (blue). -
Gene Info — PPP1CB
Entrez GeneID
5500Protein Accession#
P62140Gene Name
PPP1CB
Gene Alias
MGC3672, PP-1B, PPP1CD
Gene Description
protein phosphatase 1, catalytic subunit, beta isoform
Omim ID
600590Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
protein phosphatase 1, catalytic subunit, beta|protein phosphatase 1, catalytic subunit, delta isoform|protein phosphatase 1-beta|protein phosphatase 1-delta|serine/threonine protein phosphatase PP1-beta catalytic subunit
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Interactome
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