Ctsb recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of rat brain (A), C6 (B), NIH3T3 (C) whole cell lysates with Cathepsin B recombinant monoclonal antibody (Cat # RAB02480).
Immunofluorescence
Immunofluorescent analysis of Raw264.7 cells with Cathepsin B recombinant monoclonal antibody (Cat # RAB02480). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Ctsb.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein of mouse Cathepsin B.
Theoretical MW (kDa)
43
Reactivity
Mouse, Rat
Specificity
Recognizes endogenous levels of Cathepsin B protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of rat brain (A), C6 (B), NIH3T3 (C) whole cell lysates with Cathepsin B recombinant monoclonal antibody (Cat # RAB02480).Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of Raw264.7 cells with Cathepsin B recombinant monoclonal antibody (Cat # RAB02480). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. -
Gene Info — Ctsb
Entrez GeneID
13030Protein Accession#
P10605;P00787Gene Name
Ctsb
Gene Alias
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Gene Description
cathepsin B
Gene Ontology
HyperlinkOther Designations
CATHEPSIN B PRECURSOR (CATHEPSIN B1)
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