Products

Western Blot (Cell lysate)

Western blot analysis of K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates with PKA C alpha recombinant monoclonal antibody (Cat # RAB02476).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical analysis of human colon cancer formalin fixed paraffin embedded tissue section using PKA C alpha recombinant monoclonal antibody (Cat # RAB02476). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.54). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunocytochemistry

Immunofluorescence

Immunofluorescent analysis of HeLa cells with PKA C alpha recombinant monoclonal antibody (Cat # RAB02476). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Immunoprecipitation

Entrez GeneID

5566

Protein Accession#

P17612

Gene Name

PRKACA

Gene Alias

MGC102831, MGC48865, PKACA

Gene Description

protein kinase, cAMP-dependent, catalytic, alpha

Omim ID

601639

Gene Ontology

Hyperlink

Gene Summary

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq

Other Designations

PKA C-alpha|cAMP-dependent protein kinase catalytic subunit alpha|cAMP-dependent protein kinase catalytic subunit alpha, isoform 1|protein kinase A catalytic subunit