PRKACA monoclonal antibody, clone 7H3A4
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: PRKACA-hIgGFc transfected HEK293 cell with PRKACA monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PRKACA monoclonal antibody, clone 7H3A4.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human PRKACA.
Immunogen
Recombinant protein corresponding to amino acid 1-120 of human PRKACA from E. coli.
Host
Mouse
Theoretical MW (kDa)
40.6
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: HEK293 cell; Lane 2: PRKACA-hIgGFc transfected HEK293 cell with PRKACA monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of PRKACA monoclonal antibody, clone 7H3A4. -
Gene Info — PRKACA
Entrez GeneID
5566Gene Name
PRKACA
Gene Alias
MGC102831, MGC48865, PKACA
Gene Description
protein kinase, cAMP-dependent, catalytic, alpha
Omim ID
601639Gene Ontology
HyperlinkGene Summary
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
PKA C-alpha|cAMP-dependent protein kinase catalytic subunit alpha|cAMP-dependent protein kinase catalytic subunit alpha, isoform 1|protein kinase A catalytic subunit
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Interactome
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Pathway
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