MAP3K2 recombinant monoclonal antibody, clone R06-2K7
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MAP3K2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human MAP3K2.
Theoretical MW (kDa)
Calculated MW: 70 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence(1:50-1:200)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: C6 and Lane 3: 3T3 lysates with MAP3K2 recombinant monoclonal antibody, clone R06-2K7 (Cat # RAB02108).Immunocytochemistry
Immunocytochemical staining of Hela with MAP3K2 recombinant monoclonal antibody, clone R06-2K7 (Cat # RAB02108). (A) MAP3K2 (green) and (B) DAPI (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — MAP3K2
Entrez GeneID
10746Protein Accession#
Q9Y2U5Gene Name
MAP3K2
Gene Alias
MEKK2, MEKK2B
Gene Description
mitogen-activated protein kinase kinase kinase 2
Omim ID
609487Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. [provided by RefSeq
Other Designations
MAP/ERK kinase kinase 2|MAPK/ERK kinase kinase 2|MEK kinase 2
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Interactome
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