EGLN3 recombinant monoclonal antibody, clone R04-3K9
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: rat lung and Lane 2: mouse lung lysates with EGLN3 recombinant monoclonal antibody, clone R04-3K9 (Cat # RAB02004).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human colon cancer with EGLN3 recombinant monoclonal antibody, clone R04-3K9 (Cat # RAB02004).
High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EGLN3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human EGLN3.
Theoretical MW (kDa)
Calculated MW: 27 kD
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: rat lung and Lane 2: mouse lung lysates with EGLN3 recombinant monoclonal antibody, clone R04-3K9 (Cat # RAB02004).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human colon cancer with EGLN3 recombinant monoclonal antibody, clone R04-3K9 (Cat # RAB02004).
High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunoprecipitation
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Gene Info — EGLN3
Entrez GeneID
112399Protein Accession#
Q9H6Z9Gene Name
EGLN3
Gene Alias
FLJ21620, HIFPH3, MGC125998, MGC125999, PHD3
Gene Description
egl nine homolog 3 (C. elegans)
Omim ID
606426Gene Ontology
HyperlinkOther Designations
HIF prolyl hydroxylase 3|egl nine homolog 3|egl nine-like protein 3 isoform
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Interactome
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Pathway
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Disease
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