SAFB recombinant monoclonal antibody, clone R03-7A1
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More Files
- More Functions
Western Blot
Western blot analysis of rat brain lysates with SAFB recombinant monoclonal antibody, clone R03-7A1 (Cat # RAB01929).
Immunocytochemistry
Immunocytochemical staining of 293 with SAFB recombinant monoclonal antibody, clone R03-7A1 (Cat # RAB01929). (A) SAFB (green) and (B) DAPI (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SAFB.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human SAFB.
Theoretical MW (kDa)
Calculated MW: 103 k
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of rat brain lysates with SAFB recombinant monoclonal antibody, clone R03-7A1 (Cat # RAB01929).Immunohistochemistry
Immunocytochemistry
Immunocytochemical staining of 293 with SAFB recombinant monoclonal antibody, clone R03-7A1 (Cat # RAB01929). (A) SAFB (green) and (B) DAPI (blue).Immunofluorescence
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Gene Info — SAFB
Entrez GeneID
6294Protein Accession#
Q15424Gene Name
SAFB
Gene Alias
DKFZp779C1727, HAP, HET, SAFB1
Gene Description
scaffold attachment factor B
Omim ID
602895Gene Ontology
HyperlinkGene Summary
This gene encodes a DNA-binding protein that has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. This encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of the heat shock protein 27 transcription and also can act as an estrogen receptor corepressor. This gene is a candidate gene for breast tumorigenesis. [provided by RefSeq
Other Designations
HSP27 ERE-TATA-binding protein|Hsp27 ERE-TATA binding protein|glutathione S-transferase fusion protein|heat-shock protein (HSP27) estrogen response element and TATA box-binding protein|scaffold attachment factor B1
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