UBE2D3 recombinant monoclonal antibody, clone R01-4J4
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More Files
- More Functions
Western Blot
Western blot analysis of Lane1: K562, Lane2: rat brain, Lane3: C6, Lane4: 3T3 and Lane5: Hela lysates with UBE2D3 recombinant monoclonal antibody, clone R01-4J4 (Cat # RAB01846).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human UBE2D3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human UBE2D3.
Theoretical MW (kDa)
Calculated MW: 17 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1: K562, Lane2: rat brain, Lane3: C6, Lane4: 3T3 and Lane5: Hela lysates with UBE2D3 recombinant monoclonal antibody, clone R01-4J4 (Cat # RAB01846).Immunoprecipitation
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Gene Info — UBE2D3
Entrez GeneID
7323Protein Accession#
P61077Gene Name
UBE2D3
Gene Alias
E2(17)KB3, MGC43926, MGC5416, UBC4/5, UBCH5C
Gene Description
ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
Omim ID
602963Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000161758|OTTHUMP00000161759|ubiquitin carrier protein|ubiquitin-conjugating enzyme E2-17 kDa 3|ubiquitin-conjugating enzyme E2D 3|ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5)
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Interactome
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Pathway
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Disease
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