PRKACA recombinant monoclonal antibody, clone R07-8H3
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More Files
- More Functions
Western Blot
Western blot analysis of cAMP Protein Kinase Catalytic subunit in K562, C6, 3T3, Hela lysates using human cAMP Protein Kinase Catalytic subunit recombinant monoclonal antibody, clone R07-8H3 (Cat # RAB01541).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human cAMP Protein Kinase Catalytic subunit.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human cAMP Protein Kinase Catalytic subunit
Theoretical MW (kDa)
Calculated MW: 41 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1/100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of cAMP Protein Kinase Catalytic subunit in K562, C6, 3T3, Hela lysates using human cAMP Protein Kinase Catalytic subunit recombinant monoclonal antibody, clone R07-8H3 (Cat # RAB01541).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunoprecipitation
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Gene Info — PRKACA
Entrez GeneID
5566Protein Accession#
P17612Gene Name
PRKACA
Gene Alias
MGC102831, MGC48865, PKACA
Gene Description
protein kinase, cAMP-dependent, catalytic, alpha
Omim ID
601639Gene Ontology
HyperlinkGene Summary
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
PKA C-alpha|cAMP-dependent protein kinase catalytic subunit alpha|cAMP-dependent protein kinase catalytic subunit alpha, isoform 1|protein kinase A catalytic subunit
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Interactome
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Pathway
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