WT1 recombinant monoclonal antibody, clone WT1/1434R
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human WT1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human WT1.
Reactivity
Human
Specificity
Recognizes a 47-55kDa tumor suppressor protein which identified as WT1 protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm's tumors.
Form
Liquid
Purification
Protein A purification
Isotype
IgG, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/million cells)
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS (0.05% BSA and 0.05% azide)
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human kidney with WT1 recombinant monoclonal antibody, clone WT1/1434R (Cat # RAB00291).Immunofluorescence
Flow Cytometry
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Gene Info — WT1
Entrez GeneID
7490Protein Accession#
P19544Gene Name
WT1
Gene Alias
GUD, WAGR, WIT-2, WT33
Gene Description
Wilms tumor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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The WT1 Wilms tumor gene product: a developmentally regulated transcription factor in the kidney that functions as a tumor suppressor.
Rauscher FJ 3rd.
FASEB Journal 1993 Jul; 7(10):896.
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The WT1 Wilms tumor gene product: a developmentally regulated transcription factor in the kidney that functions as a tumor suppressor.
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