WT1 recombinant monoclonal antibody, clone RMWT1-1
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of human kidney with anti-WT1 recombinant monoclonal antibody, clone RMWT1-1 (Cat # RAB03835).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against recombinant human WT1 protein.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to recombinant human WT1 protein
Reactivity
Human
Form
Liquid
Conjugation
Unconjugated
Purification
Protein A affinity chromatography
Concentration
0.2 mg/mL
Isotype
IgG
Recommend Usage
Immunofluorescence (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(0.5-1 ug/mL for 30 min at RT)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 0.1 mg/ml BSA, 0.05% sodium azide
Storage Instruction
Store at 2~8°C.
Aliquot to avoid repeated freezing and thawing.Note
Optimal dilutions for each application to be determined by the researcher
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of human kidney with anti-WT1 recombinant monoclonal antibody, clone RMWT1-1 (Cat # RAB03835).Immunofluorescence
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Gene Info — WT1
Entrez GeneID
7490Protein Accession#
P19544Gene Name
WT1
Gene Alias
GUD, WAGR, WIT-2, WT33
Gene Description
Wilms tumor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq
Other Designations
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Interactome
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Disease
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