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Last updated: 2016/12/4
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TUBA1B polyclonal antibody

  • Catalog # : PAB9934
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of TUBA1B.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 427-441 of human TUBA1B.
  • Host:
  • Rabbit
  • Reactivity:
  • Birds, Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:25000
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using TUBA1B polyclonal antibody (Cat # PAB9934) shows detection of a predominant band at ~50 KDa corresponding to TUBA1B (arrowhead).
    Lanes correspond to whole cell lysates from mouse brain (Lane 1), rat brain (Lane 2), A-431 cells (Lane 3), Jurkat cells (Lane 4) and HeLa cells (Lane 5).
    Approximately 35 &mciro;g of each lysate was separated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1 : 1,200.
    Reaction occurred 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rb IgG [H&L] MX10 for 45 min at room temperature.
    IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Immunofluorescence
  • ELISA
  • Application Image
  • Immunofluorescence
  • ELISA
  • Gene Information
  • Protein Accession#:
  • P68363;NP006000
  • Gene Name:
  • TUBA1B
  • Gene Alias:
  • K-ALPHA-1
  • Gene Description:
  • tubulin, alpha 1b
  • Other Designations:
  • alpha tubulin,tubulin, alpha, ubiquitous
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