TUBA1B polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot using TUBA1B polyclonal antibody (Cat # PAB9934) shows detection of a predominant band at ~50 KDa corresponding to TUBA1B (arrowhead).
Lanes correspond to whole cell lysates from mouse brain (Lane 1), rat brain (Lane 2), A-431 cells (Lane 3), Jurkat cells (Lane 4) and HeLa cells (Lane 5).
Approximately 35 &mciro;g of each lysate was separated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 1,200.
Reaction occurred 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rb IgG [H&L] MX10 for 45 min at room temperature.
IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TUBA1B.
Immunogen
A synthetic peptide corresponding to amino acids 427-441 of human TUBA1B.
Host
Rabbit
Reactivity
Birds, Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:25000
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using TUBA1B polyclonal antibody (Cat # PAB9934) shows detection of a predominant band at ~50 KDa corresponding to TUBA1B (arrowhead).
Lanes correspond to whole cell lysates from mouse brain (Lane 1), rat brain (Lane 2), A-431 cells (Lane 3), Jurkat cells (Lane 4) and HeLa cells (Lane 5).
Approximately 35 &mciro;g of each lysate was separated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 1,200.
Reaction occurred 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rb IgG [H&L] MX10 for 45 min at room temperature.
IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — TUBA1B
Entrez GeneID
10376Protein Accession#
P68363;NP006000Gene Name
TUBA1B
Gene Alias
K-ALPHA-1
Gene Description
tubulin, alpha 1b
Omim ID
602530Gene Ontology
HyperlinkGene Summary
O
Other Designations
alpha tubulin|tubulin, alpha, ubiquitous
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Interactome
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Pathway
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Publication Reference
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Structural features and restricted expression of a human alpha-tubulin gene.
Hall JL, Cowan NJ.
Nucleic Acids Research 1985 Jan; 13(1):207.
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Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions.
Cowan NJ, Dobner PR, Fuchs EV, Cleveland DW.
Molecular and Cellular Biology 1993 Oct; 3(10):1738.
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Structural features and restricted expression of a human alpha-tubulin gene.
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