WASL (phospho Y256) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of control (lane 1) and pervanadate-treated (lane 2) A-431 cells (20 ug/lane). Blots were probed with either WASL (phospho Y256) polyclonal antibody (Cat # PAB7899).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of WASL.
Immunogen
Synthetic phosphopeptide (conjugated with BSA) corresponding to residues surrounding Y256 of human WASL.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
The human WASP sequence has a two amino acid difference in the same region surrounding tyrosine 291.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:20000)
Western Blot (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of control (lane 1) and pervanadate-treated (lane 2) A-431 cells (20 ug/lane). Blots were probed with either WASL (phospho Y256) polyclonal antibody (Cat # PAB7899).Enzyme-linked Immunoabsorbent Assay
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Gene Info — WASL
Entrez GeneID
8976Gene Name
WASL
Gene Alias
DKFZp779G0847, MGC48327, N-WASP, NWASP
Gene Description
Wiskott-Aldrich syndrome-like
Omim ID
605056Gene Ontology
HyperlinkGene Summary
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes. [provided by RefSeq
Other Designations
Wiskott-Aldrich syndrome gene-like|Wiskott-Aldrich syndrome gene-like protein|neural Wiskott-Aldrich syndrome protein
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Interactome
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Pathway
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Publication Reference
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Focal adhesion kinase regulation of N-WASP subcellular localization and function.
Wu X, Suetsugu S, Cooper LA, Takenawa T, Guan JL.
The Journal of Biological Chemistry 2004 Mar; 279(10):9565.
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Contingent phosphorylation/dephosphorylation provides a mechanism of molecular memory in WASP.
Torres E, Rosen MK.
Molecular Cell 2003 May; 11(5):1215.
Application：WB, Recombinant protein.
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Involvement of wiskott-aldrich syndrome protein in B-cell cytoplasmic tyrosine kinase pathway.
Baba Y, Nonoyama S, Matsushita M, Yamadori T, Hashimoto S, Imai K, Arai S, Kunikata T, Kurimoto M, Kurosaki T, Ochs HD, Yata J, Kishimoto T, Tsukada S.
Blood 1999 Mar; 93(6):2003.
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Focal adhesion kinase regulation of N-WASP subcellular localization and function.
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