WASL recombinant monoclonal antibody, clone R02-7K5
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More Files
- More Functions
Western Blot
Western blot analysis of 3T3 lysates with WASL recombinant monoclonal antibody, clone R02-7K5 (Cat # RAB02042).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human colon cancer with WASL recombinant monoclonal antibody, clone R02-7K5 (Cat # RAB02042).
High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human WASL.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human WASL.
Theoretical MW (kDa)
Calculated MW: 55 kD
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of 3T3 lysates with WASL recombinant monoclonal antibody, clone R02-7K5 (Cat # RAB02042).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin/PFA-fixed paraffin-embedded sections) of human colon cancer with WASL recombinant monoclonal antibody, clone R02-7K5 (Cat # RAB02042).
High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunofluorescence
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Gene Info — WASL
Entrez GeneID
8976Protein Accession#
O00401Gene Name
WASL
Gene Alias
DKFZp779G0847, MGC48327, N-WASP, NWASP
Gene Description
Wiskott-Aldrich syndrome-like
Omim ID
605056Gene Ontology
HyperlinkGene Summary
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes. [provided by RefSeq
Other Designations
Wiskott-Aldrich syndrome gene-like|Wiskott-Aldrich syndrome gene-like protein|neural Wiskott-Aldrich syndrome protein
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Interactome
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Pathway
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