PAM polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
PAM polyclonal antibody (Cat # PAB7587) (1 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PAM.
Immunogen
A synthetic peptide corresponding to human PAM.
Sequence
C-DQEKEDDGSESEE
Host
Goat
Theoretical MW (kDa)
108, 101, 96.3, 98.8
Reactivity
Human
Specificity
This antibody is expected to recognize all reported isoforms (NP_000910.2; NP_620121.1; NP_620176.1 and NP_620177.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PAM polyclonal antibody (Cat # PAB7587) (1 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PAM
Entrez GeneID
5066Protein Accession#
NP_000910.2;NP_620121.1;NP_620176.1;NP_620177.1Gene Name
PAM
Gene Alias
PAL, PHM
Gene Description
peptidylglycine alpha-amidating monooxygenase
Omim ID
170270Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known. [provided by RefSeq
Other Designations
pancreatic peptidylglycine alpha-amidating monooxygenase|peptidyl alpha-amidating enzyme|peptidyl-alpha-hydroxyglycine alpha-amidating lyase|peptidylglycine 2-hydroxylase|peptidylglycine alpha-hydroxylating monooxygenase
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Interactome
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Disease
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Publication Reference
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Human peptidylglycine alpha-amidating monooxygenase: cDNA, cloning and functional expression of a truncated form in COS cells.
Glauder J, Ragg H, Rauch J, Engels JW.
Biochemical and Biophysical Research Communications 1990 Jun; 169(2):551.
Application：WB-Re, Human, Recombinant proteins.
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Human peptidylglycine alpha-amidating monooxygenase: cDNA, cloning and functional expression of a truncated form in COS cells.
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