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PAM polyclonal antibody

  • Catalog # : PAB7587
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of PAM.
  • Immunogen:
  • A synthetic peptide corresponding to human PAM.
  • Sequence:
  • C-DQEKEDDGSESEE
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 108, 101, 96.3, 98.8
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize all reported isoforms (NP_000910.2; NP_620121.1; NP_620176.1 and NP_620177.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:4000)
    Western Blot (0.3-1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • PAM polyclonal antibody (Cat # PAB7587) (1 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 5066
  • Protein Accession#:
  • NP_000910.2;NP_620121.1;NP_620176.1;NP_620177.1
  • Gene Name:
  • PAM
  • Gene Alias:
  • PAL,PHM
  • Gene Description:
  • peptidylglycine alpha-amidating monooxygenase
  • Gene Summary:
  • This gene encodes a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known. [provided by RefSeq
  • Other Designations:
  • pancreatic peptidylglycine alpha-amidating monooxygenase,peptidyl alpha-amidating enzyme,peptidyl-alpha-hydroxyglycine alpha-amidating lyase,peptidylglycine 2-hydroxylase,peptidylglycine alpha-hydroxylating monooxygenase
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