GCNT3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
GCNT3 polyclonal antibody (Cat # PAB7556) (0.1 ug/mL) staining of human duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
293 overexpressing GCNT3 and probed with GCNT3 polyclonal antibody (Cat # PAB7556) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GCNT3.
Immunogen
A synthetic peptide corresponding to amino acids 410-422 of human GCNT3.
Sequence
C-NKFDPKVDDNALQ
Host
Goat
Theoretical MW (kDa)
50.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GCNT3 polyclonal antibody (Cat # PAB7556) (0.1 ug/mL) staining of human duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing GCNT3 and probed with GCNT3 polyclonal antibody (Cat # PAB7556) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GCNT3
Entrez GeneID
9245Protein Accession#
NP_004742.1Gene Name
GCNT3
Gene Alias
C2/4GnT, C2GnT-M, C2GnT2, GnT-M
Gene Description
glucosaminyl (N-acetyl) transferase 3, mucin type
Omim ID
606836Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins
Other Designations
OTTHUMP00000163601|beta1,6-N-acetylglucosaminyltransferase
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Interactome
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Pathway
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Publication Reference
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Control of O-glycan branch formation. Molecular cloning and characterization of a novel thymus-associated core 2 beta1, 6-n-acetylglucosaminyltransferase.
Schwientek T, Yeh JC, Levery SB, Keck B, Merkx G, van Kessel AG, Fukuda M, Clausen H.
The Journal of Biological Chemistry 2000 Apr; 275(15):11106.
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Control of O-glycan branch formation. Molecular cloning and characterization of a novel thymus-associated core 2 beta1, 6-n-acetylglucosaminyltransferase.
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