ASS1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
ASS1 polyclonal antibody (Cat # PAB7517) (0.03 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ASS1 polyclonal antibody (Cat # PAB7517)(2.5 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ASS1.
Immunogen
A synthetic peptide corresponding to internal region of human ASS1.
Sequence
C-ENPKNQAPPGLYTKTQD
Host
Goat
Theoretical MW (kDa)
46.5
Reactivity
Bovine, Human
Specificity
The variants represent identical protein (NP_000041.2 and NP_446464.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.01-0.03 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ASS1 polyclonal antibody (Cat # PAB7517) (0.03 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ASS1 polyclonal antibody (Cat # PAB7517)(2.5 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ASS1
Entrez GeneID
445Protein Accession#
NP_000041.2;NP_446464.1Gene Name
ASS1
Gene Alias
ASS, CTLN1
Gene Description
argininosuccinate synthetase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000022362|OTTHUMP00000022363|OTTHUMP00000022364|citrulline--aspartate ligase
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Interactome
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Pathway
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Disease
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Publication Reference
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Phosphorylation of argininosuccinate synthase by protein kinase A.
Corbin KD, Pendleton LC, Solomonson LP, Eichler DC.
Biochemical and Biophysical Research Communications 2008 Oct; 377(4):1042.
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Phosphorylation of argininosuccinate synthase by protein kinase A.
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