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Last updated: 2017/2/26
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ASS1 polyclonal antibody

  • Catalog # : PAB7517
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of ASS1.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human ASS1.
  • Sequence:
  • C-ENPKNQAPPGLYTKTQD
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 46.5
  • Reactivity:
  • Bovine, Human
  • Specificity:
  • The variants represent identical protein (NP_000041.2 and NP_446464.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:64000)
    Western Blot (0.01-0.03 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • ASS1 polyclonal antibody (Cat # PAB7517) (0.03 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • ASS1 polyclonal antibody (Cat # PAB7517)(2.5 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • Immunofluorescence
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunofluorescence
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 445
  • Protein Accession#:
  • NP_000041.2;NP_446464.1
  • Gene Name:
  • ASS1
  • Gene Alias:
  • ASS,CTLN1
  • Gene Description:
  • argininosuccinate synthetase 1
  • Gene Summary:
  • The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000022362,OTTHUMP00000022363,OTTHUMP00000022364,citrulline--aspartate ligase
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