RPE65 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
RPE65 polyclonal antibody (Cat # PAB7451) (0.5 ug/mL) staining of rat retina lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RPE65.
Immunogen
A synthetic peptide corresponding to human RPE65.
Sequence
C-EVKKNARKAPQPE
Host
Goat
Theoretical MW (kDa)
60.9
Reactivity
Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
RPE65 polyclonal antibody (Cat # PAB7451) (0.5 ug/mL) staining of rat retina lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RPE65
Entrez GeneID
6121Protein Accession#
NP_000320.1Gene Name
RPE65
Gene Alias
LCA2, RP20, mRPE65, rd12, sRPE65
Gene Description
retinal pigment epithelium-specific protein 65kDa
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa. [provided by RefSeq
Other Designations
OTTHUMP00000010788|RBP-binding membrane protein|p63|retinal pigment epithelium-specific 61 kDa protein|retinal pigment epithelium-specific protein (65kD)|retinitis pigmentosa 20 (autosomal recessive)
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Interactome
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Pathway
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Disease
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Publication Reference
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Gene therapeutic prospects in early onset of severe retinal dystrophy: restoration of vision in RPE65 Briard dogs using an AAV serotype 4 vector that specifically targets the retinal pigmented epithelium.
Rolling F, Le Meur G, Stieger K, Smith AJ, Weber M, Deschamps JY, Nivard D, Mendes-Madeira A, Provost N, Pereon Y, Cherel Y, Ali RR, Hamel C, Moullier P, Rolling F.
Bull Mem Acad R Med Belg 2006 Jan; 161(10-12):497.
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Gene therapeutic prospects in early onset of severe retinal dystrophy: restoration of vision in RPE65 Briard dogs using an AAV serotype 4 vector that specifically targets the retinal pigmented epithelium.
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