LASP1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of LASP1.
Immunogen
A synthetic peptide corresponding to human LASP1.
Sequence
C-PERRDSQDGSSYR
Host
Goat
Theoretical MW (kDa)
29.7
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
LASP1 polyclonal antibody (Cat # PAB7399) (0.1 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — LASP1
Entrez GeneID
3927Protein Accession#
NP_006139.1Gene Name
LASP1
Gene Alias
Lasp-1, MLN50
Gene Description
LIM and SH3 protein 1
Omim ID
602920Gene Ontology
HyperlinkGene Summary
This gene encodes a member of a LIM protein subfamily characterized by a LIM motif and a domain of Src homology region 3. The encoded protein functions as an actin-binding protein and possibly in cytoskeletal organization. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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Silencing of LASP-1 influences zyxin localization, inhibits proliferation and reduces migration in breast cancer cells.
Grunewald TG, Kammerer U, Schulze E, Schindler D, Honig A, Zimmer M, Butt E.
Experimental Cell Research 2006 Apr; 312(7):974.
Application:IF, IHC-P, WB-Tr, Human, BT-20 cells, Human breast cancer, HUVECs.
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Silencing of LASP-1 influences zyxin localization, inhibits proliferation and reduces migration in breast cancer cells.
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