Cyld polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of Cyld.
Immunogen
A synthetic peptide corresponding to mouse Cyld.
Sequence
C-DSQQSKSKNPWYIDE
Host
Goat
Theoretical MW (kDa)
107
Reactivity
Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The Cyld polyclonal antibody (Cat # PAB7243) (0.5 ug/mL) staining of rat brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Cyld
Entrez GeneID
74256Protein Accession#
NP_775545.1Gene Name
Cyld
Gene Alias
2010013M14Rik, 2900009M21Rik, C130039D01Rik, CDMT, CYLD1, EAC, mKIAA0849
Gene Description
cylindromatosis (turban tumor syndrome)
Gene Ontology
HyperlinkOther Designations
ubiquitin carboxyl-terminal hydrolase CYLD
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Publication Reference
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Cyld inhibits tumor cell proliferation by blocking Bcl-3-dependent NF-kappaB signaling.
Massoumi R, Chmielarska K, Hennecke K, Pfeifer A, Fassler R.
Cell 2006 May; 125(4):665.
Application:IHC-P, WB, Mouse, Brain, MEFs, Skin.
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Cyld inhibits tumor cell proliferation by blocking Bcl-3-dependent NF-kappaB signaling.
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