EPHB2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
EPHB2 polyclonal antibody (Cat # PAB7121) (1 ug/mL) staining of human lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of EPHB2.
Immunogen
A synthetic peptide corresponding to human EPHB2.
Sequence
C-QKDRNHRPKFGQ
Host
Goat
Theoretical MW (kDa)
110
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms ( NP_059145.2 and NP_004433.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
EPHB2 polyclonal antibody (Cat # PAB7121) (1 ug/mL) staining of human lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EPHB2
Entrez GeneID
2048Protein Accession#
NP_004433.2;NP_059145.2Gene Name
EPHB2
Gene Alias
CAPB, DRT, EPHT3, ERK, Hek5, MGC87492, PCBC, Tyro5
Gene Description
EPH receptor B2
Gene Ontology
HyperlinkGene Summary
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq
Other Designations
OTTHUMP00000002914|OTTHUMP00000002916|developmentally-regulated eph-related tyrosine kinase|elk-related tyrosine kinase|eph tyrosine kinase 3|ephrin receptor EphB2|prostate cancer-brain cancer susceptibility
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Interactome
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Pathway
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Disease
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Publication Reference
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EphB2 regulates axonal growth at the midline in the developing auditory brainstem.
Cramer KS, Cerretti DP, Siddiqui SA.
Developmental Biology 2006 Jul; 295(1):76.
Application：IF, IHC-Fr, Chicken, Chicken embryos.
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EphB2 regulates axonal growth at the midline in the developing auditory brainstem.
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