EPHB2 monoclonal antibody, clone 48CT12.6.4
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis of EPHB2 recombinant protein reacted with EPHB2 monoclonal antibody (Cat # MAB12325) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EPHB2 monoclonal antibody (Cat # MAB12325) at 1:200 dilution.
Flow Cytometry
Flow cytometric analysis of HepG2 cells (bottom histogram) and a negative control cell (top histogram) reacted with EPHB2 monoclonal antibody (Cat # MAB12325) at 1:10-1:50 dilution.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human EPHB2.
Immunogen
Recombinant His fusion protein corresponding to amino acids 127~425 of human EPHB2.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (1:10-1:50)
Immunohistochemistry (1:200)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of EPHB2 recombinant protein reacted with EPHB2 monoclonal antibody (Cat # MAB12325) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EPHB2 monoclonal antibody (Cat # MAB12325) at 1:200 dilution.Flow Cytometry
Flow cytometric analysis of HepG2 cells (bottom histogram) and a negative control cell (top histogram) reacted with EPHB2 monoclonal antibody (Cat # MAB12325) at 1:10-1:50 dilution. -
Gene Info — EPHB2
Entrez GeneID
2048Gene Name
EPHB2
Gene Alias
CAPB, DRT, EPHT3, ERK, Hek5, MGC87492, PCBC, Tyro5
Gene Description
EPH receptor B2
Gene Ontology
HyperlinkGene Summary
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq
Other Designations
OTTHUMP00000002914|OTTHUMP00000002916|developmentally-regulated eph-related tyrosine kinase|elk-related tyrosine kinase|eph tyrosine kinase 3|ephrin receptor EphB2|prostate cancer-brain cancer susceptibility
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Interactome
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Pathway
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Disease
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