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Last updated: 2016/12/4
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GPX4 polyclonal antibody

  • Catalog # : PAB7065
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of GPX4.
  • Immunogen:
  • A synthetic peptide corresponding to C-terminus of human GPX4.
  • Sequence:
  • C-EEPLVIEKDLPHY
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 22.1
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize isoform A (NP_002076.2) and isoform C (NP_0010.34937.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:16000)
    Western blot (0.1-0.3 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • GPX4 polyclonal antibody (Cat # PAB7065) (0.1 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • GPX4 polyclonal antibody (Cat # PAB7065, 0.3 ug/mL) staining of paraffin embedded human testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2879
  • Protein Accession#:
  • NP_002076.2;NP_0010.34937.1
  • Gene Name:
  • GPX4
  • Gene Alias:
  • MCSP,PHGPx,snGPx,snPHGPx
  • Gene Description:
  • glutathione peroxidase 4 (phospholipid hydroperoxidase)
  • Gene Summary:
  • Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. [provided by RefSeq
  • Other Designations:
  • glutathione peroxidase 4,phospholipid hydroperoxidase,phospholipid hydroperoxide glutathione peroxidase, mitochondrial,sperm nucleus glutathione peroxidase
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