GPX4 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GPX4.
Immunogen
A synthetic peptide corresponding to C-terminus of human GPX4.
Sequence
C-EEPLVIEKDLPHY
Host
Goat
Theoretical MW (kDa)
22.1
Reactivity
Human
Specificity
This antibody is expected to recognize isoform A (NP_002076.2) and isoform C (NP_0010.34937.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GPX4 polyclonal antibody (Cat # PAB7065) (0.1 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
GPX4 polyclonal antibody (Cat # PAB7065, 0.3 ug/mL) staining of paraffin embedded human testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GPX4
Entrez GeneID
2879Protein Accession#
NP_002076.2;NP_0010.34937.1Gene Name
GPX4
Gene Alias
MCSP, PHGPx, snGPx, snPHGPx
Gene Description
glutathione peroxidase 4 (phospholipid hydroperoxidase)
Omim ID
138322Gene Ontology
HyperlinkGene Summary
Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. [provided by RefSeq
Other Designations
glutathione peroxidase 4|phospholipid hydroperoxidase|phospholipid hydroperoxide glutathione peroxidase, mitochondrial|sperm nucleus glutathione peroxidase
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Interactome
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Pathway
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Disease
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Publication Reference
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Mammalian selenoprotein thioredoxin-glutathione reductase. Roles in disulfide bond formation and sperm maturation.
Su D, Novoselov SV, Sun QA, Moustafa ME, Zhou Y, Oko R, Hatfield DL, Gladyshev VN.
The Journal of Biological Chemistry 2005 Jul; 280(28):26491.
Application:WB-Ti, Mouse, Testes, Sperm.
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Mammalian selenoprotein thioredoxin-glutathione reductase. Roles in disulfide bond formation and sperm maturation.
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