ACSL5 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
ACSL5 polyclonal antibody (Cat # PAB7030) (1 ug/mL) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ACSL5.
Immunogen
A synthetic peptide corresponding to human ACSL5.
Sequence
C-RTQIDSLYEHIQD
Host
Goat
Theoretical MW (kDa)
82.3, 76
Reactivity
Human
Specificity
This antibody is expected to recognize isoform a (NP_057318.2) and isoform b (NP_976313.1 and NP_976314.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ACSL5 polyclonal antibody (Cat # PAB7030) (1 ug/mL) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACSL5
Entrez GeneID
51703Protein Accession#
NP_057318.2;NP_976313.1;NP_976314.1Gene Name
ACSL5
Gene Alias
ACS2, ACS5, FACL5
Gene Description
acyl-CoA synthetase long-chain family member 5
Omim ID
605677Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
FACL5 for fatty acid coenzyme A ligase 5|OTTHUMP00000020489|OTTHUMP00000020490|fatty acid coenzyme A ligase 5|fatty-acid-Coenzyme A ligase, long-chain 5|long-chain acyl-CoA synthetase 5|long-chain fatty acid coenzyme A ligase 5
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Interactome
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Pathway
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Disease
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Publication Reference
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Coeliac disease is associated with impaired expression of acyl-CoA-synthetase 5.
Obermuller N, Keith M, Kopitz J, Autschbach F, Schirmacher P, Gassler N.
International Journal of Colorectal Disease 2006 Mar; 21(2):130.
Application：IHC-P, WB-Ti, Human, Small intestinal, Large intestinal.
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Coeliac disease is associated with impaired expression of acyl-CoA-synthetase 5.
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