ACSL5 monoclonal antibody, clone CL0275
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with ACSL5 monoclonal antibody, clone CL0275 (Cat # MAB15587).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human duodenum with ACSL5 monoclonal antibody, clone CL0275 (Cat # MAB15587) shows strong cytoplasmic and membrane immunoreactivity in the glandular cells.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human ACSL5.
Immunogen
Recombinant protein corresponding to human ACSL5.
Epitope
This antibody binds to an epitope located within the peptide sequence MDPFDDDLKQ as determined by overlapping synthetic peptides.
Sequence
VHIVNKADIAMVICDTPQKALVLIGNVEKGFTPSLKVIILMDPFDDDLKQRGEKSGIEILSLYDAENLGKEHFRKPVPPSPEDLSVICFTSGTTGDPK
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with ACSL5 monoclonal antibody, clone CL0275 (Cat # MAB15587).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human duodenum with ACSL5 monoclonal antibody, clone CL0275 (Cat # MAB15587) shows strong cytoplasmic and membrane immunoreactivity in the glandular cells. -
Gene Info — ACSL5
Entrez GeneID
51703Protein Accession#
Q9ULC5Gene Name
ACSL5
Gene Alias
ACS2, ACS5, FACL5
Gene Description
acyl-CoA synthetase long-chain family member 5
Omim ID
605677Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
FACL5 for fatty acid coenzyme A ligase 5|OTTHUMP00000020489|OTTHUMP00000020490|fatty acid coenzyme A ligase 5|fatty-acid-Coenzyme A ligase, long-chain 5|long-chain acyl-CoA synthetase 5|long-chain fatty acid coenzyme A ligase 5
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Interactome
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Pathway
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Disease
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