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Last updated: 2017/2/19
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AMPH polyclonal antibody

  • Catalog # : PAB7026
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of AMPH.
  • Immunogen:
  • A synthetic peptide corresponding to human AMPH.
  • Sequence:
  • C-KRKDESRISKAE
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 76.3, 71.9
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_001626.1 and NP_647477.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:16000)
    Western blot (0.1-0.5 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • AMPH polyclonal antibody (Cat # PAB7026) (0.5 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • AMPH polyclonal antibody (Cat # PAB7026, 2.5 ug/mL) staining of paraffin embedded human cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 273
  • Protein Accession#:
  • NP_001626.1;NP_647477.1
  • Gene Name:
  • AMPH
  • Gene Alias:
  • AMPH1
  • Gene Description:
  • amphiphysin
  • Gene Summary:
  • This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. [provided by RefSeq
  • Other Designations:
  • Stiff-Man syndrome with breast cancer 128kDa autoantigen,amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)
  • Related Disease
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