AMPH polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
AMPH polyclonal antibody (Cat # PAB7026) (0.5 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
AMPH polyclonal antibody (Cat # PAB7026, 2.5 ug/mL) staining of paraffin embedded human cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AMPH.
Immunogen
A synthetic peptide corresponding to human AMPH.
Sequence
C-KRKDESRISKAE
Host
Goat
Theoretical MW (kDa)
76.3, 71.9
Reactivity
Human, Mouse
Specificity
This antibody is expected to recognize both reported isoforms (NP_001626.1 and NP_647477.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western blot (0.1-0.5 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
AMPH polyclonal antibody (Cat # PAB7026) (0.5 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
AMPH polyclonal antibody (Cat # PAB7026, 2.5 ug/mL) staining of paraffin embedded human cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — AMPH
Entrez GeneID
273Protein Accession#
NP_001626.1;NP_647477.1Gene Name
AMPH
Gene Alias
AMPH1
Gene Description
amphiphysin
Omim ID
600418Gene Ontology
HyperlinkGene Summary
This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. [provided by RefSeq
Other Designations
Stiff-Man syndrome with breast cancer 128kDa autoantigen|amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen)
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Paraneoplastic stiff-person syndrome: passive transfer to rats by means of IgG antibodies to amphiphysin.
Sommer C, Weishaupt A, Brinkhoff J, Biko L, Wessig C, Gold R, Toyka KV.
The Lancet 2005 Apr; 365(9468):1406.
Application：ELISA, IHC-Fr, WB, Human, Rat, Human cerebrospinal fluid, Serum, Rat brains, Rat plasma, Rat spinal cord.
-
Paraneoplastic stiff-person syndrome: passive transfer to rats by means of IgG antibodies to amphiphysin.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com