RNF115 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RNF115.
Immunogen
A synthetic peptide corresponding to human RNF115.
Sequence
C-DQDNRANERGHQTH
Host
Goat
Theoretical MW (kDa)
33.7
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
RNF115 polyclonal antibody (Cat # PAB6979) (0.3 ug/mL) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RNF115
Entrez GeneID
27246Protein Accession#
NP_055270.1Gene Name
RNF115
Gene Alias
BCA2, ZNF364
Gene Description
ring finger protein 115
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000015602|Rabring 7|zinc finger protein 364
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Interactome
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Disease
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Publication Reference
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A novel RING-type ubiquitin ligase breast cancer-associated gene 2 correlates with outcome in invasive breast cancer.
Burger AM, Gao Y, Amemiya Y, Kahn HJ, Kitching R, Yang Y, Sun P, Narod SA, Hanna WM, Seth AK.
Cancer Research 2005 Nov; 65(22):1040.
Application:IP, IS, IHC-P, WB-Ce, WB-Tr, Human, MCF-7, T47D cells, Breast tumors.
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A novel RING-type ubiquitin ligase breast cancer-associated gene 2 correlates with outcome in invasive breast cancer.
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