PPP2R1A polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
PPP2R1A polyclonal antibody (Cat # PAB6772) (0.03 ug/mL) staining of human lymph node lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PPP2R1A.
Immunogen
A synthetic peptide corresponding to human PPP2R1A.
Sequence
C-KYFAQEALTVLSLA
Host
Goat
Theoretical MW (kDa)
65.3
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PPP2R1A polyclonal antibody (Cat # PAB6772) (0.03 ug/mL) staining of human lymph node lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPP2R1A
Entrez GeneID
5518Protein Accession#
NP_055040.2Gene Name
PPP2R1A
Gene Alias
MGC786, PR65A
Gene Description
protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform
Omim ID
605983Gene Ontology
HyperlinkGene Summary
This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. [provided by RefSeq
Other Designations
PP2A, subunit A, PR65-alpha isoform|PP2A, subunit A, R1-alpha isoform|alpha isoform of regulatory subunit A, protein phosphatase 2|medium tumor antigen-associated 61 KDA protein|protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isof
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Interactome
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Pathway
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Disease
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Publication Reference
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Regulation of protein phosphatase 2A activity by heat shock transcription factor 2.
Hong Y, Sarge KD.
The Journal of Biological Chemistry 1999 May; 274(19):12967.
Application：WB-Ce, WB-Re, Human, HeLa, K-562 cells, Recombinant protein.
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Regulation of protein phosphatase 2A activity by heat shock transcription factor 2.
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