HNF4A polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of HNF4A.
Immunogen
A synthetic peptide corresponding to human HNF4A.
Sequence
RLSKTLVDMDMADY-C
Host
Goat
Theoretical MW (kDa)
51.6, 52.8, 46.6
Reactivity
Human
Specificity
This antibody is expected to recognize the reported isoforms a, b and c (NP_849180.1, NP_000448.3, NP_849181.1 resp.).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
HNF4A polyclonal antibody (Cat # PAB6768) (0.1 ug/mL) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HNF4A
Entrez GeneID
3172Protein Accession#
NP_849180.1;NP_000448.3;NP_849181.1Gene Name
HNF4A
Gene Alias
FLJ39654, HNF4, HNF4a7, HNF4a8, HNF4a9, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14
Gene Description
hepatocyte nuclear factor 4, alpha
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq
Other Designations
HNF4-alpha|OTTHUMP00000031060|OTTHUMP00000031062|hepatic nuclear factor 4 alpha|hepatocyte nuclear factor 4 alpha|transcription factor-14
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Interactome
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Pathway
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Disease
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Publication Reference
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Polymorphisms in both promoters of hepatocyte nuclear factor 4-alpha are associated with type 2 diabetes in the Amish.
Damcott CM, Hoppman N, Ott SH, Reinhart LJ, Wang J, Pollin TI, O'Connell JR, Mitchell BD, Shuldiner AR.
Diabetes 2004 Dec; 53(12):3337.
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Polymorphisms in both promoters of hepatocyte nuclear factor 4-alpha are associated with type 2 diabetes in the Amish.
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