HNF4A recombinant monoclonal antibody, clone 10A7
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: HL-60 whole cell lysate; Lane 2: Rat Liver tissue lysate.
Immunohistochemistry
Immunohistochemistry image of HNF4A recombinant monoclonal antibody, clone 10A7 diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of MCF-7 Cells with HNF4A recombinant monoclonal antibody, clone 10A7 at 1:20, counter-stained with DAPI.
Flow Cytometry
Overlay Peak curve showing HepG2 cells stained with HNF4A recombinant monoclonal antibody, clone 10A7 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and rat HNF4A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HNF4A.
Theoretical MW (kDa)
Calculated MW: 52
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry(1:50-1:200)
Immunohistochemistry(1:50-1:200)
Immunofluorescence(1:20-1:200)
Western Blot(1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: HL-60 whole cell lysate; Lane 2: Rat Liver tissue lysate.Immunohistochemistry
Immunohistochemistry image of HNF4A recombinant monoclonal antibody, clone 10A7 diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of MCF-7 Cells with HNF4A recombinant monoclonal antibody, clone 10A7 at 1:20, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay Peak curve showing HepG2 cells stained with HNF4A recombinant monoclonal antibody, clone 10A7 (red line) at 1:50. -
Gene Info — HNF4A
Entrez GeneID
3172Protein Accession#
P41235Gene Name
HNF4A
Gene Alias
FLJ39654, HNF4, HNF4a7, HNF4a8, HNF4a9, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14
Gene Description
hepatocyte nuclear factor 4, alpha
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq
Other Designations
HNF4-alpha|OTTHUMP00000031060|OTTHUMP00000031062|hepatic nuclear factor 4 alpha|hepatocyte nuclear factor 4 alpha|transcription factor-14
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Interactome
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Pathway
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Disease
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