ARPC3 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
ARPC3 polyclonal antibody (Cat # PAB6621) staining (1 ug/mL) of Mouse brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ARPC3.
Immunogen
A synthetic peptide corresponding to human ARPC3.
Sequence
C-RQFMNKSLSGPGQ
Host
Goat
Theoretical MW (kDa)
20.5
Reactivity
Human, Mouse
Specificity
There are two hypothetical proteins called "similar to the Arp2/3 protein complex subunit p21-Arc" that are virtually identical (CAC14083, XP_208062).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ARPC3 polyclonal antibody (Cat # PAB6621) staining (1 ug/mL) of Mouse brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ARPC3
Entrez GeneID
10094Protein Accession#
NP_005710Gene Name
ARPC3
Gene Alias
ARC21, p21-Arc
Gene Description
actin related protein 2/3 complex, subunit 3, 21kDa
Omim ID
604225Gene Ontology
HyperlinkGene Summary
This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p21 subunit, has yet to be determined. [provided by RefSeq
Other Designations
ARP2/3 protein complex subunit p21|actin related protein 2/3 complex subunit 3|actin related protein 2/3 complex, subunit 3 (21 kD)
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Interactome
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Pathway
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Publication Reference
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The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.
Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ.
The Journal of Cell Biology 1997 Jul; 138(2):375.
Application：WB-Ce, Chicken, Human, Mouse, 3T3, HeLa cells, Fibroblast.
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The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.
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