WDFY1 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
WDFY1 polyclonal antibody (Cat # PAB6612) (4 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of WDFY1.
Immunogen
A synthetic peptide corresponding to C-terminus of human WDFY1.
Sequence
VVGCSLATGFSPH
Host
Goat
Theoretical MW (kDa)
46.3
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (as represented by NP_065881 and NP_848127)
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
WDFY1 polyclonal antibody (Cat # PAB6612) (4 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — WDFY1
Entrez GeneID
57590Protein Accession#
NP_065881;NP_848127Gene Name
WDFY1
Gene Alias
FENS-1, WDF1, ZFYVE17
Gene Description
WD repeat and FYVE domain containing 1
Gene Ontology
HyperlinkGene Summary
The FYVE domain mediates the recruitment of proteins involved in membrane trafficking and cell signaling to phosphatidylinositol 3-phosphate (PtdIns(3)P)-containing membranes. This gene encodes a protein which contains a single FYVE domain and multiple WD40 repeats. This protein is localized to endosomes. [provided by RefSeq
Other Designations
WD40 and FYVE domain containing 1|phosphoinositide-binding protein SR1
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Interactome
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Publication Reference
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FENS-1 and DFCP1 are FYVE domain-containing proteins with distinct functions in the endosomal and Golgi compartments.
Ridley SH, Ktistakis N, Davidson K, Anderson KE, Manifava M, Ellson CD, Lipp P, Bootman M, Coadwell J, Nazarian A, Erdjument-Bromage H, Tempst P, Cooper MA, Thuring JW, Lim ZY, Holmes AB, Stephens LR, Hawkins PT.
Journal of Cell Science 2001 Nov; 114(Pt 22):3991.
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FENS-1 and DFCP1 are FYVE domain-containing proteins with distinct functions in the endosomal and Golgi compartments.
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