FANCG polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
FANCG polyclonal antibody (Cat # PAB6485) staining (0.5 ug/mL) of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing FANCG and probed with FANCG polyclonal antibody (Cat # PAB6485) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FANCG.
Immunogen
A synthetic peptide corresponding to human FANCG.
Sequence
LEEFRTSLPKSCDL
Host
Goat
Theoretical MW (kDa)
68.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
FANCG polyclonal antibody (Cat # PAB6485) staining (0.5 ug/mL) of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing FANCG and probed with FANCG polyclonal antibody (Cat # PAB6485) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FANCG
Entrez GeneID
2189Protein Accession#
NP_004620.1Gene Name
FANCG
Gene Alias
FAG, XRCC9
Gene Description
Fanconi anemia, complementation group G
Omim ID
602956Gene Ontology
HyperlinkGene Summary
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G. [provided by RefSeq
Other Designations
DNA repair protein XRCC9|OTTHUMP00000021319|X-ray repair complementing defective repair in Chinese hamster cells 9|X-ray repair, complementing defective, in Chinese hamster, 9
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Interactome
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Disease
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Publication Reference
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The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells.
Liu N, Lamerdin JE, Tucker JD, Zhou ZQ, Walter CA, Albala JS, Busch DB, Thompson LH.
PNAS 1997 Aug; 94(17):9232.
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The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells.
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